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Functional identification of a novel transport system for endogenous and synthetic opioid peptides in the rabbit conjunctival epithelial cell line CJVE.
- Source :
-
Pharmaceutical research [Pharm Res] 2009 May; Vol. 26 (5), pp. 1226-35. Date of Electronic Publication: 2008 Sep 10. - Publication Year :
- 2009
-
Abstract
- Purpose: To investigate whether conjunctival epithelial cells express transport processes for opioid peptides.<br />Methods: We monitored the uptake of [(3)H]deltorphin II and [(3)H]DADLE, two hydrolysis-resistant synthetic opioid peptides, in the rabbit conjunctival epithelial cell line CJVE and elucidated the characteristics of the uptake process.<br />Results: CJVE cells express robust uptake activity for deltorphin II and DADLE. Both opioid peptides compete with each other for transport. Several endogenous and synthetic opioid peptides, but not non-peptide opioid antagonists, are recognized by the transport process. Though various peptides inhibit the uptake of deltorphin II and DADLE in a similar manner, the uptake of deltorphin II is partly Na(+)-dependent whereas that of DADLE mostly Na(+)-independent. The transport process shows high affinity for many endogenous/synthetic opioid peptides. Functional features reveal that this transport process may be distinct from the opioid peptide transport system described in the retinal pigment epithelial cell line ARPE-19 and also from the organic anion transporting polypeptides, which are known to transport opioid peptides.<br />Conclusions: CJVE cells express a novel, hitherto unknown transport process for endogenous/synthetic opioid peptides. This new transport process may offer an effective delivery route for opioid peptide drugs to the posterior segment of the eye.
- Subjects :
- Amiloride analogs & derivatives
Amiloride pharmacology
Animals
Cell Line
Enkephalin, Leucine-2-Alanine pharmacokinetics
Naloxone pharmacology
Oligopeptides pharmacokinetics
Organic Anion Transporters antagonists & inhibitors
Rabbits
Sodium-Potassium-Exchanging ATPase antagonists & inhibitors
Biological Transport drug effects
Conjunctiva cytology
Epithelial Cells metabolism
Opioid Peptides pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1573-904X
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Pharmaceutical research
- Publication Type :
- Academic Journal
- Accession number :
- 18781380
- Full Text :
- https://doi.org/10.1007/s11095-008-9709-x