Back to Search
Start Over
Pathogenicity and vaccine efficacy of different clades of Asian H5N1 avian influenza A viruses in domestic ducks.
- Source :
-
Journal of virology [J Virol] 2008 Nov; Vol. 82 (22), pp. 11374-82. Date of Electronic Publication: 2008 Sep 10. - Publication Year :
- 2008
-
Abstract
- Waterfowl represent the natural reservoir of all subtypes of influenza A viruses, including H5N1. Ducks are especially considered major contributors to the spread of H5N1 influenza A viruses because they exhibit diversity in morbidity and mortality. Therefore, as a preventive strategy against endemic as well as pandemic influenza, it is important to reduce the spread of H5N1 influenza A viruses in duck populations. Here, we describe the pathogenicity of dominant clades (clades 1 and 2) of H5N1 influenza A viruses circulating in birds in Asia. Four representatives of dominant clades of the viruses cause symptomatic infection but lead to different profiles of lethality in domestic ducks. We also demonstrate the efficacy, cross-protectiveness, and immunogenicity of three different inactivated oil emulsion whole-virus H5 influenza vaccines (derived by implementing reverse genetics) to the viruses in domestic ducks. A single dose of the vaccines containing 1 mug of hemagglutinin protein provides complete protection against a lethal A/Duck/Laos/25/06 (H5N1) influenza virus challenge, with no evidence of morbidity, mortality, or shedding of the challenge virus. Moreover, two of the three vaccines achieved complete cross-clade or cross-subclade protection against the heterologous avian influenza virus challenge. Interestingly, the vaccines induce low or undetectable titers of hemagglutination inhibition (HI), cross-HI, and/or virus neutralization antibodies. The mechanism of complete protection in the absence of detectable antibody responses remains an open question.
- Subjects :
- Adjuvants, Immunologic administration & dosage
Animals
Animals, Domestic
Antibodies, Viral blood
Asia
Cloaca virology
Ducks
Hemagglutination Inhibition Tests
Oils administration & dosage
Survival Analysis
Trachea virology
Vaccines, Inactivated immunology
Virus Shedding
Influenza A Virus, H5N1 Subtype immunology
Influenza A Virus, H5N1 Subtype pathogenicity
Influenza Vaccines immunology
Influenza in Birds prevention & control
Influenza in Birds virology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 82
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 18786988
- Full Text :
- https://doi.org/10.1128/JVI.01176-08