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Short-term dynamics of bacterial communities in a tidally affected coastal ecosystem.
- Source :
-
FEMS microbiology ecology [FEMS Microbiol Ecol] 2008 Nov; Vol. 66 (2), pp. 306-19. Date of Electronic Publication: 2008 Sep 20. - Publication Year :
- 2008
-
Abstract
- Tidal effects on the composition of free-living (FL) and particle-associated (PA) bacterial communities were studied in a tidal flat ecosystem in the southern North Sea. Denaturing gradient gel electrophoresis targeting the 16S rRNA gene and the 16S rRNA of Bacteria, Bacteroidetes, Alphaproteobacteria and the Roseobacter clade was applied. Despite strong tidal variations in the quantity and, depending on the season, also the quality of suspended matter as well as variations in bacterial activity, the bacterial community composition remained rather stable. FISH showed some variations of the community composition, but these were not related to typical tidal situations. Variations were higher during tidal cycles in May and July compared with November. Bacteroidetes, Alpha- and Gammaproteobacteria constituted the majority of the bacterial communities but relative proportions of the different groups varied considerably. On particles, Betaproteobacteria were also detected to substantial proportions. The Roseobacter clade constituted up to 90% of FL but only 30% of PA Alphaproteobacteria. Banding patterns of the Bacteroidetes-specific amplicons, and in particular those targeting the 16S rRNA, revealed tidally induced effects, as several bands appeared or disappeared at distinct events such as slack water or resuspension. Sequencing of prominent bands revealed predominantly phylotypes reported previously from this ecosystem.
- Subjects :
- Actinobacteria classification
Actinobacteria genetics
Actinobacteria growth & development
Bacteria genetics
Bacteroidetes classification
Bacteroidetes genetics
Bacteroidetes growth & development
Cloning, Molecular
DNA, Bacterial analysis
DNA, Bacterial isolation & purification
Electrophoresis, Agar Gel
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Particulate Matter
Proteobacteria classification
Proteobacteria genetics
Proteobacteria growth & development
Seasons
Sequence Analysis, DNA
Bacteria classification
Bacteria growth & development
Ecosystem
Seawater microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-6496
- Volume :
- 66
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEMS microbiology ecology
- Publication Type :
- Academic Journal
- Accession number :
- 18811653
- Full Text :
- https://doi.org/10.1111/j.1574-6941.2008.00573.x