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[Down-regulation of survivin in growth inhibition of hepatoma cells induced by a selective cyclooxygenase-2 inhibitor].
- Source :
-
The Korean journal of hepatology [Korean J Hepatol] 2008 Sep; Vol. 14 (3), pp. 351-9. - Publication Year :
- 2008
-
Abstract
- Background/aims: Cyclooxygenase-2 (COX-2) inhibitors reportedly inhibit the growth of hepatocellular carcinoma (HCC) via caspase-dependent or caspase-independent apoptosis, which is due to COX-2 being associated with hepatocarcinogenesis. Survivin is highly expressed in most human cancers, but the mechanism regulating survivin expression remains unclear. We investigated the regulatory expression of survivin in selective-COX-2-inhibitor-induced growth inhibition of hepatoma cells.<br />Methods: After treatment with NS-398 (a selective COX-2 inhibitor) at various concentrations (10, 50, 100, 150, and 200 micrometer), the growth inhibition of Hep3B hepatoma cells was assessed by an MTT cell-viability assay, DNA fragmentation gel analysis, and flow cytometry. The expression of survivin transcript was analyzed by reverse-transcription polymerase chain reactions.<br />Results: NS-398 inhibited the growth of hepatoma cells by an amount dependent on the concentration and the time since treatment. Apoptotic DNA ladder and flow-cytometry shifting to the sub-G1 phase were revealed in NS-398-induced growth inhibition of hepatoma cells. NS-398 suppressed the expression of the survivin gene in a concentration- and time-dependent manner.<br />Conclusions: Survivin was down-regulated in the growth inhibition of hepatoma cells induced by a selective COX-2 inhibitor, NS-398, in a concentration- and time-dependent manner. These results suggest the therapeutic inhibition of COX-2 via suppression of survivin in HCC.
- Subjects :
- Carcinoma, Hepatocellular enzymology
Carcinoma, Hepatocellular pathology
Cell Line, Tumor
Cell Proliferation drug effects
Cyclooxygenase 2 Inhibitors chemistry
G1 Phase
Humans
Inhibitor of Apoptosis Proteins
Liver Neoplasms enzymology
Liver Neoplasms pathology
Microtubule-Associated Proteins metabolism
Neoplasm Proteins metabolism
Nitrobenzenes chemistry
Reverse Transcriptase Polymerase Chain Reaction
Sulfonamides chemistry
Survivin
Time Factors
Carcinoma, Hepatocellular metabolism
Cyclooxygenase 2 Inhibitors pharmacology
Liver Neoplasms metabolism
Microtubule-Associated Proteins antagonists & inhibitors
Neoplasm Proteins antagonists & inhibitors
Nitrobenzenes pharmacology
Sulfonamides pharmacology
Subjects
Details
- Language :
- Korean
- ISSN :
- 1738-222X
- Volume :
- 14
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Korean journal of hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 18815458
- Full Text :
- https://doi.org/10.3350/kjhep.2008.14.3.351