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[Isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2008 May; Vol. 31 (5), pp. 753-6. - Publication Year :
- 2008
-
Abstract
- Objective: To study the methods of extraction, isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans (AEG).<br />Methods: AEG was purified by distilled water extraction, ethanol fractionation, Sephadex column chromatography. The purity and molecular weight and concentration of AEG were measured by HPLC; We divided the experiment into Physiological Saline group and the other eight groups with different doses of AEG, established RT-PRC method to observe the anti-HPV effect after their actions on the verruca tissues.<br />Results: Using HPLC, the group of AEG was divided into two glycosaminoglycans with different molecular weight as 27336 and 1152. Bioassay results showed that AEG had anti-HPV-DNA activity, the lowest effective concentration was 0.781 mg/ml.<br />Conclusion: AEG extracted by this method is a mixture with two molecular weight glycosaminoglycans, and has obvious anti-HPV effect.
- Subjects :
- Chromatography, High Pressure Liquid
DNA, Viral drug effects
DNA, Viral isolation & purification
Glycosaminoglycans chemistry
Human papillomavirus 6 isolation & purification
Hydrogen-Ion Concentration
Molecular Weight
Technology, Pharmaceutical methods
Boraginaceae chemistry
Glycosaminoglycans isolation & purification
Glycosaminoglycans pharmacology
Human papillomavirus 6 drug effects
Plants, Medicinal chemistry
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 31
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 18826155