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[HtpG protein of Shigella flexneri 2a strain 2457T evokes inflammatory response in mice].
- Source :
-
Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2008 Jul; Vol. 48 (7), pp. 905-10. - Publication Year :
- 2008
-
Abstract
- Objective: To analyze the function of htpG of S. flexneri 2a 2457T, we constructed an htpG deletion mutant and a recovery mutant.<br />Methods: gamma-Red recombination system was used to construct an htpG deletion mutant of S. flexneri 2a 2457T. In addition, a recover mutant was obtained by introducing a low-copy plasmid containing one copy of htpG gene into the deletion mutant. Then, the growth curves of wild-type strain, deletion mutant and recover mutant were measured. Some of biochemical tests were also investigated. Furthermore, the Sereny tests were performed to evaluate the virulence of these strains.<br />Results: No significant difference were observed among three strains. However, the titers of some inflammatory factors evoked by wild-type strain, deletion mutant and recovery mutant in intraperitoneal injected mice were quite different.<br />Conclusion: These results suggest that the HtpG protein of Shigella flexneri 2a strain 2457T might be involved in the immunopathogenesis.
- Subjects :
- Animals
Bacterial Proteins genetics
Enzyme-Linked Immunosorbent Assay
Female
HSP90 Heat-Shock Proteins deficiency
HSP90 Heat-Shock Proteins genetics
Inflammation blood
Interferon-gamma blood
Interleukin-1 blood
Mice
Mice, Inbred BALB C
Sequence Deletion
Tumor Necrosis Factor-alpha blood
Bacterial Proteins immunology
HSP90 Heat-Shock Proteins immunology
Inflammation immunology
Shigella flexneri classification
Shigella flexneri immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0001-6209
- Volume :
- 48
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Wei sheng wu xue bao = Acta microbiologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 18837368