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[Imatinib induces c-kit positive myeloma cells apoptosis].
- Source :
-
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi [Zhonghua Xue Ye Xue Za Zhi] 2008 Apr; Vol. 29 (4), pp. 230-3. - Publication Year :
- 2008
-
Abstract
- Objective: To explore the influence of Imatinib on multiple myeloma cells expressing c-kit in vitro and its mechanism.<br />Methods: KM3 cells were treated with Imatinib at different concentrations, and cell growth index were evaluated by XTT assay, cell cycle by flow cytometry, apoptosis by Annexin V/ PI and DNA ladder, and change in protein level by Western blot.<br />Results: Imatinib inhibited proliferation of KM3 cells at concentrations more than 0.25 micromol/L in a dose-dependent manner, and the 48 h IC50 was 0.33 micromol/L (P < 0.01). Imatinib arrested cell in C0/G1 phase. Annexin V/PI staining and DNA ladder indicated that Imatinib had a substantial effect on inducing apoptosis of KM3 cells in a dose-dependent manner and induced pro-caspase-3 and poly ADP-ribose polymerase (PARP) cleaved. Imatinib inhibited expression of c-kit and provoked a decrease of IL-6 induced c-kit phosphorylation in vitro.<br />Conclusion: Imatinib inhibits KM3 cells proliferation and induces the cells apoptosis by inhibiting c-kit signalling transduction.
- Subjects :
- Benzamides
Cell Cycle drug effects
Cell Differentiation drug effects
Cell Line, Tumor
Humans
Imatinib Mesylate
Multiple Myeloma metabolism
Piperazines administration & dosage
Proto-Oncogene Proteins c-kit metabolism
Pyrimidines administration & dosage
Apoptosis drug effects
Multiple Myeloma pathology
Piperazines pharmacology
Pyrimidines pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0253-2727
- Volume :
- 29
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
- Publication Type :
- Academic Journal
- Accession number :
- 18843975