Chemiluminescence flow-through sensor for ofloxacin using solid-phase PbO(2) as an oxidant.

A novel chemiluminscence (CL) flow-through sensor for ofloxacin is described. It was based on the sensitizing effect of ofloxacin on the CL oxidation of sulfite by PbO(2) in H(2)SO(4) media. By a very simply means, the solid-phase PbO(2) was immobilized inside of the CL flow cell as CL oxidant. The column of solid PbO(2) could be reused about 400 times during a period of 50 h. The calibration graph is linear in the range 0.2-10 mug ml(-1) with a detection limit of 7.8x10(-8) g ml(-1) (S/N=3). This method has been successfully applied to determine ofloxacin in pharmaceutical preparation.