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Enhancement of preneoplastic lesion yield by Chios Mastic Gum in a rat liver medium-term carcinogenesis bioassay.
- Source :
-
Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2009 Jan 01; Vol. 234 (1), pp. 135-42. Date of Electronic Publication: 2008 Oct 15. - Publication Year :
- 2009
-
Abstract
- The mastic (Pistacia lentiscus var. chia) tree is native throughout the Mediterranean region and has long proved a source of food additives and medical treatments. To investigate the modifying effects of Chios Mastic Gum on rat liver carcinogenesis, 6-week-old male F344 rats were subjected to the established rat liver medium-term carcinogenesis bioassay (Ito-test). At the commencement, rats (groups 1-4) were intraperitoneally injected with 200 mg/kg body weight of diethylnitrosamine (DEN). After two weeks, mastic was added to CRF (Charles River Formula)-1 powdered basal diet at doses of 0, 0.01, 0.1 and 1% in groups 1-4, respectively. At week 3, all rats were underwent two-thirds partial hepatectomy. The experiment was terminated at week 8. As results show, liver weights were significantly increased in a mastic dose-dependent manner among groups 1-4. The numbers (/cm(2)) and the areas (mm(2)/cm(2)) of glutathione S-transferase placental form (GST-P)-positive cell foci (>or=0.2 mm in diameter) were significantly increased in the DEN-1% group compared to the DEN-alone group, along with the average areas per foci and larger-sized foci (>or=0.4 mm). 5-Bromo-2'-deoxyuridine (BrdU)+GST-P double-immunohistochemistry showed the highest BrdU-labeling indices within GST-P foci in the DEN-1% group. 8-hydroxydeoxyguanosine (8-OHdG) levels in liver DNA did not vary, while real-time quantitative polymerase chain reaction (PCR) analysis of livers revealed many up- or down-regulated genes in the DEN-1% group. In conclusion, this is the first report to display a promotion potential of Chios Mastic Gum on the formation of preneoplastic lesions in the established rat liver medium-term carcinogenesis bioassay.
- Subjects :
- 8-Hydroxy-2'-Deoxyguanosine
Animals
Carcinogenicity Tests
DNA metabolism
Deoxyguanosine analogs & derivatives
Deoxyguanosine metabolism
Diethylnitrosamine
Dose-Response Relationship, Drug
Glutathione S-Transferase pi metabolism
Immunohistochemistry methods
Liver metabolism
Male
Mastic Resin
Mediterranean Region
Organ Size drug effects
Polymerase Chain Reaction
Rats
Rats, Inbred F344
Resins, Plant administration & dosage
Gene Expression Regulation drug effects
Liver drug effects
Pistacia chemistry
Precancerous Conditions chemically induced
Resins, Plant toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0333
- Volume :
- 234
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Toxicology and applied pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 18977376
- Full Text :
- https://doi.org/10.1016/j.taap.2008.10.001