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Characterization of shedding patterns of Porcine circovirus types 2a and 2b in experimentally inoculated mature boars.
- Source :
-
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2008 Nov; Vol. 20 (6), pp. 725-34. - Publication Year :
- 2008
-
Abstract
- Porcine circovirus-2 (PCV-2) is an economically important swine pathogen and causes PCV-associated disease (PCVAD) in pigs worldwide. Currently, 2 genotypes of PCV-2, PCV-2a and -2b, are circulating in U.S. swine herds. The objectives of the current study were to evaluate the amount of PCV-2 DNA present in semen over time, compare and correlate incidence and amount of PCV-2 present in semen samples to that present in serum samples and blood swabs, and determine if there are differences in shedding patterns between PCV-2a and -2b. Fifteen 7-month-old PCV-2-naïve Landrace boars (Sus scrofa) were randomly allocated to 3 treatment groups. The boars in group 1 (n = 3) served as negative controls, and those in groups 2 (n = 6) and 3 (n = 6) were intranasally and intramuscularly inoculated with PCV-2a and -2b, respectively. Semen, serum, and blood swab samples were collected up to 90 days postinoculation (DPI), and necropsies were performed on DPI 23, 48, and 90. Larger quantities of both PCV-2a and -2b DNA were detected earlier in serum and blood swab samples than in raw semen of experimentally inoculated boars. The incidence and duration of presence of PCV-2 DNA in semen varied among boars; however, intermittent shedding was not observed. In all sex glands, PCV-2 DNA was detected by polymerase chain reaction; however, PCV-2 antigen was not detected by immunohistochemistry, and PCV-2 had no effect on sperm morphology. Differences in shedding patterns between PCV-2a and -2b were not observed under the study conditions.
- Subjects :
- Animals
Blood virology
Circoviridae Infections veterinary
DNA, Single-Stranded genetics
DNA, Single-Stranded isolation & purification
DNA, Viral genetics
DNA, Viral isolation & purification
Genome, Viral
Male
Polymerase Chain Reaction
Semen virology
Swine Diseases virology
Circovirus isolation & purification
Swine virology
Virus Shedding physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1040-6387
- Volume :
- 20
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Publication Type :
- Academic Journal
- Accession number :
- 18987221
- Full Text :
- https://doi.org/10.1177/104063870802000603