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Identification of novel synthetic peptide showing angiogenic activity in human endothelial cells.

Authors :
Lee CH
Lee MS
Kim SJ
Je YT
Ryu SH
Lee T
Source :
Peptides [Peptides] 2009 Feb; Vol. 30 (2), pp. 409-18. Date of Electronic Publication: 2008 Oct 17.
Publication Year :
2009

Abstract

A novel synthetic hexapeptide (SFKLRY-NH(2)) that displays angiogenic activity has been identified by positional scanning of a synthetic peptide combinatorial library (PS-SPCL). The peptide induced proliferation, migration, and capillary-like tube formation in primary cultured HUVECs, and augmented vessel sprouting ex vivo while attenuated by the treatment with pertussis toxin (PTX) or U73122 (PLC-inhibitor) suggesting the influence of PTX-sensitive G-proteins and PLC. In addition, SFKLRY-NH(2) up-regulated the expression of VEGF-A in HUVECs and the neutralizing antibody against VEGF suppressed SFKLRY-NH(2)-induced tube formation activity. Taken together, these results suggest that SFKLRY-NH(2) may induce blood vessel formation by PTX-sensitive G protein-coupled receptor-PLC-Ca(2+) signaling cascade leading into VEGF-A expression in HUVECs.

Details

Language :
English
ISSN :
0196-9781
Volume :
30
Issue :
2
Database :
MEDLINE
Journal :
Peptides
Publication Type :
Academic Journal
Accession number :
18992291
Full Text :
https://doi.org/10.1016/j.peptides.2008.10.003