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Modulation of potassium ion channel proteins utilising antibodies.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2008; Vol. 491, pp. 247-55. - Publication Year :
- 2008
-
Abstract
- The application of antibodies to living cells has the potential to modulate the function of specific proteins by virtue of their high specificity. This specificity has proven effective in determining the involvement of many proteins in neuronal function where specific agonists and antagonists do not exist, e.g. ion channel subunits. We discuss a way to utilise subunit specific antibodies to target individual channel subunits in electrophysiological experiments to determine functional roles within native neurones. Utilising this approach, we have investigated the role of the voltage-gated potassium channel Kv3.1b subunit within a region of the brainstem important in the regulation of autonomic function. We provide some useful control experiments in order to help validate this method. We conclude that antibodies can be extremely valuable in determining the functions of specific proteins in living neurones in neuroscience research.
- Subjects :
- Animals
Antibody Specificity
Immunohistochemistry
Patch-Clamp Techniques
Protein Subunits immunology
Rats
Action Potentials physiology
Antibodies
Brain Stem physiology
Nerve Tissue Proteins immunology
Nerve Tissue Proteins physiology
Neurons physiology
Shaw Potassium Channels immunology
Shaw Potassium Channels physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1064-3745
- Volume :
- 491
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 18998098
- Full Text :
- https://doi.org/10.1007/978-1-59745-526-8_19