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Crystallization and preliminary X-ray studies of a Bacillus subtilis and Thermus thermophilus HB8 chimeric 3-isopropylmalate dehydrogenase.

Authors :
Onodera K
Moriyama H
Takenaka A
Tanaka N
Akutsu N
Muro M
Oshima T
Imada K
Sato M
Katsube Y
Source :
Journal of biochemistry [J Biochem] 1991 Jan; Vol. 109 (1), pp. 1-2.
Publication Year :
1991

Abstract

A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3(1)21 or P3(2)21, a = b = 77.1 A and c = 158.3 A, which is isomorphous with that of the native enzyme from T. thermophilus.

Details

Language :
English
ISSN :
0021-924X
Volume :
109
Issue :
1
Database :
MEDLINE
Journal :
Journal of biochemistry
Publication Type :
Academic Journal
Accession number :
1901851
Full Text :
https://doi.org/10.1093/oxfordjournals.jbchem.a123328