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SiRNA inhibition of ER-alpha expression reduces KGF-induced proliferation of breast cancer cells.
- Source :
-
Anticancer research [Anticancer Res] 2008 Sep-Oct; Vol. 28 (5A), pp. 2733-5. - Publication Year :
- 2008
-
Abstract
- Background: Keratinocyte growth factor (KGF) produces a rapid increase in the proliferation and motility of estrogen receptor (ER)-positive breast cancer cells which is abolished by estrogen deprivation and/or anti-estrogen treatment. The present study examined the hypothesis that ER-alpha is involved in the KGF proliferation in MCF-7 cancer cells using small interfering RNA (siRNA) to selectively inhibit ER-alpha expression.<br />Materials and Methods: At 48 hours following ER-alpha siRNA transfection, the MCF-7 cells were treated with KGF (50 ng/ml) or vehicle for 24 hours. Cell proliferation was measured using a MTT assay. ER-alpha protein levels were quantified by Western blotting.<br />Results: ER-alpha siRNA transfection significantly reduced ER-alpha expression and MCF-7 cell proliferation. KGF-mediated enhancement of cell proliferation and motile cell morphology were reduced or absent in the siRNA transfected MCF-7 cells.<br />Conclusion: ER-alpha expression is associated with KGF-induced proliferation of breast cancer cells.
- Subjects :
- Breast Neoplasms metabolism
Breast Neoplasms pathology
Cell Growth Processes drug effects
Cell Growth Processes genetics
Cell Line, Tumor
Estrogen Receptor alpha biosynthesis
Estrogen Receptor alpha genetics
Fibroblast Growth Factor 7 antagonists & inhibitors
Humans
Transfection
Breast Neoplasms genetics
Estrogen Receptor alpha antagonists & inhibitors
Fibroblast Growth Factor 7 pharmacology
RNA, Small Interfering genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0250-7005
- Volume :
- 28
- Issue :
- 5A
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 19035303