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Functional improvement of infarcted heart by co-injection of embryonic stem cells with temperature-responsive chitosan hydrogel.
- Source :
-
Tissue engineering. Part A [Tissue Eng Part A] 2009 Jun; Vol. 15 (6), pp. 1437-47. - Publication Year :
- 2009
-
Abstract
- Transplantation of embryonic stem cells (ESCs) can improve cardiac function in treatment of myocardial infarction. The low rate of cell retention and survival within the ischemic tissues makes the application of cell transplantation techniques difficult. In this study, we used a temperature-responsive chitosan hydrogel (as scaffold) combined with ESCs to maintain viable cells in the infarcted tissue. Temperature-responsive chitosan hydrogel was prepared and injected into the infarcted heart wall of rat infarction models alone or together with mouse ESCs. The result showed that the 24-h cell retention and 4 week graft size of both groups was significantly greater than with a phosphate buffered saline control. After 4 weeks of implantation, heart function, wall thickness, and microvessel densities within the infarct area improved in the chitosan + ESC, chitosan, and ESC group more than the PBS control. Of the three groups, the chitosan + ESC performed best. Results of this study indicate that temperature-responsive chitosan hydrogel is an injectable scaffold that can be used to deliver stem cells to infarcted myocardium. It can also increase cell retention and graft size. Cardiac function is well preserved, too.
- Subjects :
- Acridine Orange metabolism
Animals
Cell Differentiation drug effects
Cell Line
Cell Survival drug effects
Embryonic Stem Cells cytology
Embryonic Stem Cells metabolism
Female
Indoles metabolism
Injections
Mice
Microvessels cytology
Microvessels drug effects
Myocardial Infarction diagnostic imaging
Myocardial Infarction pathology
Neovascularization, Physiologic drug effects
Organic Chemicals metabolism
Propidium metabolism
Rats
Rats, Sprague-Dawley
Ultrasonography
Chitosan pharmacology
Embryonic Stem Cells transplantation
Hydrogel, Polyethylene Glycol Dimethacrylate metabolism
Myocardial Infarction physiopathology
Myocardial Infarction therapy
Recovery of Function drug effects
Temperature
Subjects
Details
- Language :
- English
- ISSN :
- 1937-335X
- Volume :
- 15
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Tissue engineering. Part A
- Publication Type :
- Academic Journal
- Accession number :
- 19061432
- Full Text :
- https://doi.org/10.1089/ten.tea.2008.0143