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Inflammation and NFkappaB activation is decreased by hypothermia following global cerebral ischemia.
- Source :
-
Neurobiology of disease [Neurobiol Dis] 2009 Feb; Vol. 33 (2), pp. 301-12. Date of Electronic Publication: 2008 Nov 19. - Publication Year :
- 2009
-
Abstract
- We previously showed that hypothermia attenuates inflammation in focal cerebral ischemia (FCI) by suppressing activating kinases of nuclear factor-kappa B (NFkappaB). Here we characterize the inflammatory response in global cerebral ischemia (GCI), and the influence of mild hypothermia. Rodents were subjected to GCI by bilateral carotid artery occlusion. The inflammatory response was accompanied by microglial activation, but not neutrophil infiltration, or blood brain barrier disruption. Mild hypothermia reduced CA1 damage, decreased microglial activation and decreased nuclear NFkappaB translocation and activation. Similar anti-inflammatory effects of hypothermia were observed in a model of pure brain inflammation that does not cause brain cell death. Primary microglial cultures subjected to oxygen glucose deprivation (OGD) or stimulated with LPS under hypothermic conditions also experienced less activation and less NFkappaB translocation. However, NFkappaB regulatory proteins were not affected by hypothermia. The inflammatory response following GCI and hypothermia's anti-inflammatory mechanism is different from that observed in FCI.
- Subjects :
- Animals
Blood-Brain Barrier physiology
Carotid Arteries
Cell Nucleus metabolism
Cells, Cultured
Glucose deficiency
Hippocampus physiopathology
Inflammation physiopathology
Male
Mice
Mice, Inbred C57BL
Microglia physiology
Neutrophils physiology
Rats
Rats, Sprague-Dawley
Brain Ischemia immunology
Brain Ischemia therapy
Hypothermia, Induced
NF-kappa B metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-953X
- Volume :
- 33
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neurobiology of disease
- Publication Type :
- Academic Journal
- Accession number :
- 19063968
- Full Text :
- https://doi.org/10.1016/j.nbd.2008.11.001