Diagnosis of human immunodeficiency virus type 1 infection in infants by use of dried blood spots and an ultrasensitive p24 antigen assay.

Authors :: Cachafeiro A
Sherman GG
Sohn AH
Beck-Sague C
Fiscus SA
Source :: Journal of clinical microbiology [J Clin Microbiol] 2009 Feb; Vol. 47 (2), pp. 459-62. Date of Electronic Publication: 2008 Dec 10.
Publication Year :: 2009
Abstract: We tested 617 dried blood spots (DBS) from human immunodeficiency virus-exposed infants from five countries using an ultrasensitive p24 antigen assay (Up24). The sensitivity was 94.4% (67/71) and the specificity was 100% (431/431) for infants with DBS specimens <or=20 months old; DBS older than 30 months demonstrated only 72.2% sensitivity (39/54) (P < 0.001) but displayed 100% specificity (61/61).

Subjects :: HIV-1 isolation & purification
Humans
Infant
Infant, Newborn
Sensitivity and Specificity
Blood virology
Desiccation
HIV Core Protein p24 blood
HIV Infections diagnosis
Specimen Handling methods

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