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Recognition and repair of UV lesions in loop structures of duplex DNA by DASH-type cryptochrome.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2008 Dec 30; Vol. 105 (52), pp. 21023-7. Date of Electronic Publication: 2008 Dec 12. - Publication Year :
- 2008
-
Abstract
- DNA photolyases and cryptochromes (cry) form a family of flavoproteins that use light energy in the blue/UV-A region for the repair of UV-induced DNA lesions or for signaling, respectively. Very recently, it was shown that members of the DASH cryptochrome subclade repair specifically cyclobutane pyrimidine dimers (CPDs) in UV-damaged single-stranded DNA. Here, we report the crystal structure of Arabidopsis cryptochrome 3 with an in-situ-repaired CPD substrate in single-stranded DNA. The structure shows a binding mode similar to that of conventional DNA photolyases. Furthermore, CPD lesions in double-stranded DNA are bound and repaired with similar efficiency as in single-stranded DNA if the CPD lesion is present in a loop structure. Together, these data reveal that DASH cryptochromes catalyze light-driven DNA repair like conventional photolyases but lack an efficient flipping mechanism for interaction with CPD lesions within duplex DNA.
- Subjects :
- Arabidopsis genetics
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Cryptochromes
Crystallography, X-Ray
DNA Damage physiology
DNA Damage radiation effects
DNA Repair radiation effects
DNA, Plant genetics
DNA, Plant metabolism
DNA, Single-Stranded genetics
DNA, Single-Stranded metabolism
Deoxyribodipyrimidine Photo-Lyase genetics
Deoxyribodipyrimidine Photo-Lyase metabolism
Protein Structure, Secondary physiology
Protein Structure, Tertiary physiology
Pyrimidine Dimers metabolism
Ultraviolet Rays adverse effects
Arabidopsis enzymology
Arabidopsis Proteins chemistry
DNA Repair physiology
DNA, Plant chemistry
DNA, Single-Stranded chemistry
Deoxyribodipyrimidine Photo-Lyase chemistry
Pyrimidine Dimers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 105
- Issue :
- 52
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 19074258
- Full Text :
- https://doi.org/10.1073/pnas.0805830106