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Dilinoleoylphosphatidylcholine ameliorates scopolamine-induced impairment of spatial learning and memory by targeting alpha7 nicotinic ACh receptors.
- Source :
-
Life sciences [Life Sci] 2009 Feb 27; Vol. 84 (9-10), pp. 263-6. Date of Electronic Publication: 2008 Dec 07. - Publication Year :
- 2009
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Abstract
- Aims: The present study was conducted to understand the role of 1,2-dilynoleoyl-sn-glycero-3-phosphocholine (DLPhtCho) in cognitive functions.<br />Main Methods: Two-electrode voltage-clamp was made to Xenopus oocytes expressing rat alpha7 acetylcholine (ACh) receptors. Field excitatory postsynaptic potentials (fEPSPs) were monitored from the CA1 region of rat hippocampal slices. Water maze test was carried out to assess spatial learning and memory for rats.<br />Key Findings: In the oocyte expression system, DLPhtCho at a concentration of 10 microM potentiated ACh-evoked currents to approximately 190% of basal amplitudes 70 min after 10-min treatment. In contrast, 1-stearoyl-2-lynoleoyl-sn-glycero-3-phosphocholine (SLPhtCho), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPhtCho), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPhtCho) had no effect on the currents. DLPhtCho (10 microM) enhanced slope of fEPSPs to about 150% of basal levels at 70-min treatment, that is inhibited by alpha-bungarotoxin, an inhibitor of alpha7 ACh receptors, while no enhancement was obtained with SLPhtCho, PLPhtCho, or POPhtCho. In the water maze test, oral administration with DLPhtCho (5 mg/kg) significantly shortened the prolonged acquisition latency for rats intraperitoneally injected with scopolamine (1 mg/kg).<br />Significance: The results of the present study show that DLPhtCho improves scopolamine-induced learning and memory deficits, possibly by facilitating hippocampal synaptic transmission under the control of alpha7 ACh receptors. DLPhtCho, therefore, could be developed as a beneficial anti-dementia drug.
- Subjects :
- Animals
Male
Oocytes cytology
Oocytes physiology
Patch-Clamp Techniques
Rats
Rats, Wistar
Spatial Behavior drug effects
Xenopus laevis
alpha7 Nicotinic Acetylcholine Receptor
Cholinergic Antagonists metabolism
Cholinergic Antagonists pharmacology
Maze Learning drug effects
Memory drug effects
Phosphatidylcholines pharmacology
Receptors, Nicotinic metabolism
Scopolamine metabolism
Scopolamine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0024-3205
- Volume :
- 84
- Issue :
- 9-10
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 19100751
- Full Text :
- https://doi.org/10.1016/j.lfs.2008.12.003