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Proteomic analysis of cerebrospinal fluid from patients with idiopathic temporal lobe epilepsy.
- Source :
-
Brain research [Brain Res] 2009 Feb 19; Vol. 1255, pp. 180-9. Date of Electronic Publication: 2008 Dec 11. - Publication Year :
- 2009
-
Abstract
- Proteomic analysis of cerebrospinal fluid (CSF) from patients with temporal lobe epilepsy (TLE) and controls was carried out using two-dimensional gel electrophoresis followed by liquid chromatography electrospray ionization tandem mass spectrometry. Five protein spots showed significant differential expression (p<0.05): vitamin D-binding protein (DBP) was elevated in the CSF of TLE patients whereas cathepsin D, apolipoprotein J, Fam3c, and superoxide dismutase 1 (SOD1) were decreased in the CSF of TLE patients. Additional six protein spots presented only in the CSF of epilepsy patients were identified as tetranectin (TN), talin-2, apolipoprotein E, immunoglobulin lambda light chain (IGL@), immunoglobulin kappa variable light chain 1-5 (IGKV1-5), and procollagen C-endopeptidase enhancer 1 (PCOLCE). Expression of DBP, SOD1 and talin-2 was validated by western blot. Our results may provide better understanding of the pathophysiologic mechanisms underlying epileptogenesis and possible epilepsy biomarkers.
- Subjects :
- Adolescent
Adult
Biomarkers cerebrospinal fluid
Blotting, Western
Cathepsin D cerebrospinal fluid
Cerebrospinal Fluid chemistry
Chromatography, Liquid
Clusterin cerebrospinal fluid
Cytokines cerebrospinal fluid
Electrophoresis, Gel, Two-Dimensional
Female
Humans
Lectins, C-Type analysis
Male
Mass Spectrometry
Middle Aged
Neoplasm Proteins cerebrospinal fluid
Proteomics methods
Superoxide Dismutase-1
Young Adult
Epilepsy, Temporal Lobe cerebrospinal fluid
Superoxide Dismutase cerebrospinal fluid
Talin cerebrospinal fluid
Vitamin D-Binding Protein cerebrospinal fluid
Subjects
Details
- Language :
- English
- ISSN :
- 1872-6240
- Volume :
- 1255
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 19109932
- Full Text :
- https://doi.org/10.1016/j.brainres.2008.12.008