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Contribution of vraSR and graSR point mutations to vancomycin resistance in vancomycin-intermediate Staphylococcus aureus.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2009 Mar; Vol. 53 (3), pp. 1231-4. Date of Electronic Publication: 2009 Jan 05. - Publication Year :
- 2009
-
Abstract
- We describe here the genetic analysis of a vancomycin-susceptible Staphylococcus aureus (VSSA) strain, Mu50Omega, a strain related to vancomycin-intermediate S. aureus (VISA) strain Mu50. Using a combination of Mu50Omega whole-genome sequencing and genome engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50Omega.
- Subjects :
- Amino Acid Sequence
Anti-Bacterial Agents pharmacology
Bacterial Proteins chemistry
Base Sequence
Chromosome Walking
DNA-Binding Proteins chemistry
Genetic Engineering
Genome, Bacterial
Humans
Microbial Sensitivity Tests
Molecular Sequence Data
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Staphylococcus aureus drug effects
Staphylococcus aureus growth & development
Staphylococcus aureus metabolism
Vancomycin pharmacology
Bacterial Proteins genetics
DNA-Binding Proteins genetics
Point Mutation
Staphylococcus aureus genetics
Vancomycin Resistance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 53
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 19124662
- Full Text :
- https://doi.org/10.1128/AAC.01173-08