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Estimation of the predictive role of plasma viral load on CD4 decline in HIV-1 subtype C-infected subjects in India.
- Source :
-
Journal of acquired immune deficiency syndromes (1999) [J Acquir Immune Defic Syndr] 2009 Feb 01; Vol. 50 (2), pp. 119-25. - Publication Year :
- 2009
-
Abstract
- Background: Plasma viral load has been shown to be a meaningful prognostic marker for disease progression in untreated, HIV-1 subtype B-infected subjects in United States and Western Europe and therefore used as a prognostic marker for disease progression. Because of high expenses of commercially available viral load assays, the role of viral load in disease progression has not been evaluated in HIV-1 subtype C-infected patients in India.<br />Methods: We developed an inexpensive real-time reverse transcriptase-polymerase chain reaction assay to quantify viral load in plasma of HIV-1 subtype C-infected subjects from India and used it in a longitudinal analysis of viral load and CD4 cell number in HIV-infected subjects from Calcutta, India.<br />Results: The real-time reverse transcriptase-polymerase chain reaction assay can quantify plasma viral load with a linear range of detection from 10 to 10 HIV-1 RNA copies per input. Longitudinal analysis of viral load in a cohort of 39 subjects over an average period of approximately 3 years indicates that 1-log increase in HIV-1 RNA level was associated with a decline of 67 CD4 cell count. Furthermore, HIV-1 RNA level between 500 and 50,000 copies per milliliter would predict a 12.9% decrease in CD4 cell count per year, whereas HIV-1 RNA levels above 50,000 copies HIV-1 RNA per milliliter would predict a 25.3% decrease in CD4 cells per year. In addition, we estimated that the mean incubation period of disease development, as defined by the loss of CD4 below 200, is 8.2 years.<br />Conclusion: Our report on the level of viral load on predicting CD4 decline in Indian subjects with HIV-1 provides an additional important tool to the physicians for treating and planning a therapeutic strategy to control HIV-1 infection in India.
- Subjects :
- CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes immunology
HIV Infections virology
HIV-1 classification
HIV-1 genetics
HIV-1 isolation & purification
HIV-1 physiology
Humans
India
Predictive Value of Tests
RNA, Viral blood
Reverse Transcriptase Polymerase Chain Reaction
Viral Load
HIV Infections immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1525-4135
- Volume :
- 50
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of acquired immune deficiency syndromes (1999)
- Publication Type :
- Academic Journal
- Accession number :
- 19131898
- Full Text :
- https://doi.org/10.1097/QAI.0b013e3181911991