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AMPA reduces surface expression of NR1 through regulation of GSK3beta.
- Source :
-
Neuroreport [Neuroreport] 2009 Jan 28; Vol. 20 (2), pp. 161-5. - Publication Year :
- 2009
-
Abstract
- Emerging evidence has suggested that alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) protects neurons from glutamate-induced neurotoxicity. In this study, we examined the effect of AMPA on the cell surface expression of the N-methyl-D-aspartate (NMDA) receptor, a central player in glutamate-induced neurotoxicity, using rat cortical neurons. AMPA (10 microM, 24 h) attenuated the expression of cell surface NR1, an NMDA receptor subunit, and also inhibited glutamate-induced increases in intracellular Ca2+. SB216763, an inhibitor of glycogen synthase kinase 3beta (GSK3beta), had effects similar to those of AMPA. We have earlier shown that AMPA treatment attenuated GSK3beta activity. Our data suggest that AMPA reduces the cell surface expression of NMDA receptors through the regulation of GSK3beta and, consequently, reduces the amount of intracellular Ca2+.
- Subjects :
- Analysis of Variance
Animals
Biotinylation
Calcium metabolism
Cerebral Cortex cytology
Cerebral Cortex drug effects
Cerebral Cortex metabolism
Excitatory Amino Acid Agonists pharmacology
Glutamic Acid metabolism
Glycogen Synthase Kinase 3 antagonists & inhibitors
Glycogen Synthase Kinase 3 beta
Immunoblotting
Neurons drug effects
Rats
Glycogen Synthase Kinase 3 metabolism
Neurons metabolism
Receptors, N-Methyl-D-Aspartate metabolism
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1473-558X
- Volume :
- 20
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neuroreport
- Publication Type :
- Academic Journal
- Accession number :
- 19151600
- Full Text :
- https://doi.org/10.1097/WNR.0b013e3283118450