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[A simple method of separating outer hair cells in Guinea pig].
- Source :
-
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery [Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi] 2008 Sep; Vol. 22 (18), pp. 829-31. - Publication Year :
- 2008
-
Abstract
- Objective: In order to meet the needs of electrophysiology outer hair cells, we investigate a method to separate outer hair cells simply and effectively.<br />Method: The basal membrane was dissected combined with spiral ligament and axis of cochlea, then incubated with enzyme, and then mechanically triturated by micropipette.<br />Result: A large number of living outer hair cells were obtained. Of 80% cells could keep good condition in 6 hours.<br />Conclusion: If increasing the enzyme concentration slightly and the large part of cochlea without outer bone was enzymes, we can get living outer hair cells easily.
Details
- Language :
- Chinese
- ISSN :
- 2096-7993
- Volume :
- 22
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery
- Publication Type :
- Academic Journal
- Accession number :
- 19166028