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Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts.
- Source :
-
The Plant cell [Plant Cell] 2009 Jan; Vol. 21 (1), pp. 146-56. Date of Electronic Publication: 2009 Jan 30. - Publication Year :
- 2009
-
Abstract
- The plant-specific DYW subclass of pentatricopeptide repeat proteins has been postulated to be involved in RNA editing of organelle transcripts. We discovered that the DYW proteins CHLORORESPIRATORY REDUCTION22 (CRR22) and CRR28 are required for editing of multiple plastid transcripts but that their DYW motifs are dispensable for editing activity in vivo. Replacement of the DYW motifs of CRR22 and CRR28 by that of CRR2, which has been shown to be capable of endonucleolytic cleavage, blocks the editing activity of both proteins. In return, the DYW motifs of neither CRR22 nor CRR28 can functionally replace that of CRR2. We propose that different DYW family members have acquired distinct functions in the divergent processes of RNA maturation, including RNA cleavage and RNA editing.
- Subjects :
- Amino Acid Sequence
Arabidopsis genetics
Arabidopsis Proteins genetics
Gene Expression Regulation, Plant
Molecular Sequence Data
Mutagenesis, Insertional
Mutation
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Protein Structure, Tertiary
RNA Probes
RNA, Plant metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Chloroplasts metabolism
RNA Editing
Subjects
Details
- Language :
- English
- ISSN :
- 1040-4651
- Volume :
- 21
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 19182104
- Full Text :
- https://doi.org/10.1105/tpc.108.064667