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Human MATER localization in specific cell domains of oocytes and follicular cells.
- Source :
-
Reproductive biomedicine online [Reprod Biomed Online] 2009 Feb; Vol. 18 (2), pp. 226-34. - Publication Year :
- 2009
-
Abstract
- MATER (Maternal Antigen That Embryos Require) is an oocyte-specific protein dependent on the maternal genome and required for early embryonic development. The gene products expressed in oocytes play important roles in folliculogenesis, fertilization and pre-implantation development. The aim of this study was to characterize the localization and distribution pattern of the human MATER protein during follicular development and after ovulation, to determine its functional role. Immunocytochemistry experiments coupled with confocal and electron microscopy analysis were carried out to determine the ultrastructural localization of MATER in human ovarian tissue and in isolated oocytes, obtained during IVF procedures. Human cumulus cells were cultured, with or without cycloheximide, to confirm endogenous biosynthesis of the protein. Human MATER is detectable at the onset of the follicular maturation process, suggesting this protein has a role at earlier stages in the human compared with other mammalian species. The presence of MATER is specific to the oocyte and follicular cells that, during maturation, are spatially and functionally associated with the oocyte. The nuclear, nucleolar and mitochondrial localization hints at a possible role in RNA processing and the metabolic activity of the cell.
- Subjects :
- Cell Nucleolus metabolism
Cell Nucleus metabolism
Cells, Cultured
Female
Granulosa Cells cytology
Humans
Microscopy, Confocal
Mitochondria metabolism
Mitochondrial Proteins
Nuclear Proteins
Oocytes cytology
Ovarian Follicle cytology
Ovarian Follicle ultrastructure
RNA Processing, Post-Transcriptional physiology
Theca Cells cytology
Tissue Distribution
Autoantigens metabolism
Granulosa Cells metabolism
Oocytes metabolism
Theca Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1472-6491
- Volume :
- 18
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Reproductive biomedicine online
- Publication Type :
- Academic Journal
- Accession number :
- 19192343
- Full Text :
- https://doi.org/10.1016/s1472-6483(10)60260-x