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A novel biomaterial for osteotropic drug nanocarriers: synthesis and biocompatibility evaluation of a PLGA-ALE conjugate.
- Source :
-
Nanomedicine (London, England) [Nanomedicine (Lond)] 2009 Feb; Vol. 4 (2), pp. 161-75. - Publication Year :
- 2009
-
Abstract
- Background & Aims: Osteotropic drug-delivery systems have been proposed as a means to provide drugs with affinity to bone tissues. Drugs or proteins have been linked chemically to bone-seeking agents, such as bisphosphonates (BPs); alternatively, drug-loaded nanoparticles have been used to target specific tissues, such as tumor areas. In our current research, these approaches were merged by synthesizing a novel bone-seeking polymer conjugate, from which targetable nanoparticles can be produced.<br />Materials & Methods: An amino-BP, alendronate (ALE) was bound covalently to a biodegradable polymer, poly(lactic-co-glycolide) (PLGA), containing a free end carboxylic group. Blood compatibility and cytotoxicity were assessed in vitro.<br />Results & Discussion: By a classical solvent-evaporation method, nanoparticles with a mean size of 200-300 nm were prepared from the conjugate; sterilization was achieved by gamma-irradiation, confirming their potential as injectable drug nanocarriers. Owing to the presence of the BP residue, PLGA-ALE nanoparticles were adsorbed onto hydroxyapatite to a higher extent than pure PLGA nanoparticles. The PLGA-ALE conjugate did not induce either hemolysis or alterations of the plasmatic phase of coagulation, or cytotoxic effects on endothelial cells and trabecular osteoblasts.<br />Conclusion: The prepared conjugate represents a novel biomaterial that is able to provide nanoparticles, which can be further loaded with drugs, such as anticancer agents, and addressed to osteolytic or other bone diseases.
- Subjects :
- Cells, Cultured
Humans
Magnetic Resonance Spectroscopy
Microscopy, Electron, Scanning
Molecular Structure
Polylactic Acid-Polyglycolic Acid Copolymer
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Alendronate chemistry
Biocompatible Materials chemistry
Drug Carriers chemistry
Lactic Acid chemistry
Nanoparticles chemistry
Polyglycolic Acid chemistry
Polymers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1748-6963
- Volume :
- 4
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nanomedicine (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 19193183
- Full Text :
- https://doi.org/10.2217/17435889.4.2.161