Back to Search
Start Over
The anti-inflammatory effect of subconjunctival bevacizumab on chemically burned rat corneas.
- Source :
-
Current eye research [Curr Eye Res] 2009 Feb; Vol. 34 (2), pp. 85-91. - Publication Year :
- 2009
-
Abstract
- Purpose: To investigate the effect of bevacizumab in a model of corneal inflammation.<br />Materials and Methods: Epithelium from the cornea and limbus was completely removed using 100% alcohol in rats. Bevacizumab (1.25 mg/0.05 ml) or normal saline was subconjunctivally injected. One week later, corneas were examined and subjected to hematoxylin-eosin and immunofluorescent staining for VEGF. Expression of IL-2, IFN-gamma, IL-6, and TGF-beta 1 were analyzed by ELISA.<br />Results: Bevacizumab showed a borderline reduction in corneal neovascularization (11.0 +/- 4.8% and 18.0 +/- 10.0% in the bevacizumab and control groups, respectively; p = 0.054), while the extent of epithelialization was not affected (5.0 +/- 3.4% and 6.1 +/- 5.2% in the bevacizumab and control groups, respectively; p = 0.715). The infiltration of inflammatory cells was reduced (99.3 +/- 22.3 cells/x 400 in the bevacizumab-injected corneas and 182.3 +/- 58.0 cells/x 400 in the controls; p = 0.013). The levels of IL-2, IFN-gamma, and IL-6 were decreased in the rats with the bevacizumab injections (44 +/- 6 and 79 +/- 9 pg/ml for IL-2 in the bevacizumab-injected group and control, respectively, p = 0.025; 45 +/- 5 and 67 +/- 6 pg/ml for IFN-gamma in the bevacizumab-injected group and controls, respectively, p = 0.043; 45 +/- 6 and 75 +/- 8 pg/ml for IL-6 in the bevacizumab-injected group and controls, respectively, p = 0.030).<br />Conclusions: Bevacizumab showed a reduction in inflammatory cell infiltration and cytokines in chemically burned rat corneas.
- Subjects :
- Animals
Antibodies, Monoclonal, Humanized
Bevacizumab
Burns, Chemical pathology
Conjunctiva
Cornea pathology
Corneal Neovascularization pathology
Disease Models, Animal
Ethanol toxicity
Eye Burns pathology
Injections
Male
Rats
Solvents toxicity
Treatment Outcome
Vascular Endothelial Growth Factor A
Angiogenesis Inhibitors administration & dosage
Antibodies, Monoclonal administration & dosage
Burns, Chemical drug therapy
Cornea drug effects
Corneal Neovascularization prevention & control
Eye Burns chemically induced
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2202
- Volume :
- 34
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Current eye research
- Publication Type :
- Academic Journal
- Accession number :
- 19219678
- Full Text :
- https://doi.org/10.1080/02713680802607740