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The intron-containing hsp82 gene of the dimorphic pathogenic fungus Histoplasma capsulatum is properly spliced in severe heat shock conditions.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 1991 Nov; Vol. 11 (11), pp. 5624-30. - Publication Year :
- 1991
-
Abstract
- We have isolated and characterized a heat-inducible gene, hsp82, from the dimorphic pathogenic fungus Histoplasma capsulatum, which is a filamentous mold at 25 degrees C and a unicellular yeast at 37 degrees C. This gene, which has a high degree of homology with other members of the hsp82 gene family, is split into three exons and two introns of 122 and 86 nucleotides, respectively. Contrary to what has been demonstrated in Drosophila melanogaster, Saccharomyces cerevisiae, and other organisms, hsp82 mRNA in H. capsulatum is properly spliced during the severe heat conditions of 37 to 40 degrees C in the temperature-sensitive Downs strain. Splicing accuracy was also observed at 42 degrees C in the temperature-tolerant G222B strain, which showed no evidence of accumulation of primary transcripts. Furthermore, the intron containing the beta-tubulin gene is also properly spliced at the upper temperature range, suggesting that the lack of a block in splicing may be a general phenomenon in this organism.
- Subjects :
- Amino Acid Sequence
Base Sequence
Blotting, Southern
Cloning, Molecular
DNA, Fungal genetics
Hot Temperature
Molecular Sequence Data
Oligonucleotides
RNA, Messenger genetics
Transcription, Genetic
Tubulin genetics
Genes, Fungal
Heat-Shock Proteins genetics
Histoplasma genetics
Introns
RNA Splicing
Subjects
Details
- Language :
- English
- ISSN :
- 0270-7306
- Volume :
- 11
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 1922067
- Full Text :
- https://doi.org/10.1128/mcb.11.11.5624-5630.1991