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[Effects of Bufalin on SYK and CBL family proteins in induction of HL-60 cell apoptosis].
- Source :
-
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2009 Feb; Vol. 17 (1), pp. 65-8. - Publication Year :
- 2009
-
Abstract
- The study was aimed to explore the mechanism of SYK and CBL family of ubiquitin ligases in Bufalin-induced HL-60 cells apoptosis. Cell viability was tested by trypan blue staining and apoptosis was detected by using flow cytometry. The expressions of CBL and CBL-b and the phosphorylation of SYK were detected by using immunoprecipitation and Western blot. The results showed that Bufalin inhibited HL-60 cell proliferation in time- and dose-dependent manners. IC(50) of suppressing cell viability at 24, 48 and 72 hours were about 26.3, 7.8 and 2.0 nmol/L respectively. The high dose of bufalin already induced apoptosis of HL-60 cells at 8 hours. SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin. It is concluded that SYK activation and CBL protein down-regulation may be involved in Bufalin-induced HL-60 cell apoptosis.
- Subjects :
- Cell Proliferation drug effects
Down-Regulation
Gene Expression Regulation, Leukemic
HL-60 Cells
Humans
Signal Transduction
Syk Kinase
Apoptosis drug effects
Bufanolides pharmacology
Intracellular Signaling Peptides and Proteins metabolism
Protein-Tyrosine Kinases metabolism
Proto-Oncogene Proteins c-cbl metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1009-2137
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhongguo shi yan xue ye xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 19236749