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[Effect of palmitate on proliferation and apoptosis of INS-1 pancreatic beta-cell].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2009 Mar; Vol. 25 (3), pp. 219-21. - Publication Year :
- 2009
-
Abstract
- Aim: To investigate the effect of saturated fatty acid palmitate (PA) on cell viability of MIN6 beta-cell and the possible cell cycle pathways affected by PA.<br />Methods: MIN6 cells were synchronized at G(0); phase by serum deprivation for 36 h, and further MIN6 cells were exposed to different concentrations of PA(0.25-1.0 mmol/L, 45 min-24 h)compared with control cell treated with BSA.Cell viability was assessed by MTT colorimetric assay. The cell cycle was measured by FACS analysis, and cell cycle proteins were further detected using Western blot.<br />Results: (1)PA significantly affected the cell viability of MIN6 cells. (2)The G(0);/G(1); cell cycle arrest (n=6, P<0.05) also induced by PA, whereas MIN6 cells in S and G(2);/M phase were decreased. (3) For cell cycle proteins, PA treatment caused significant reductions in cyclin D1 and CDK4 levels, which was consistent to the cell cycle delay.<br />Conclusion: The findings thus suggest that increased PA directly affects pancreatic beta-cell proliferation, possibly by reducing the levels of cyclin D1/CDK4 in the cells, which results in arresting in progression through the G(1); phase to the S phase of the cell cycle.
- Subjects :
- Animals
Blotting, Western
Cell Cycle drug effects
Cell Division drug effects
Cell Line, Tumor
Cell Survival drug effects
Cyclin D1 metabolism
Cyclin-Dependent Kinase 4 metabolism
Dose-Response Relationship, Drug
Flow Cytometry
G2 Phase drug effects
Insulin-Secreting Cells metabolism
Mice
S Phase drug effects
Time Factors
Apoptosis drug effects
Cell Proliferation drug effects
Insulin-Secreting Cells drug effects
Palmitates pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 25
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 19257985