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Cigarette smoke suppresses in vitro allergic activation of mouse mast cells.
- Source :
-
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology [Clin Exp Allergy] 2009 May; Vol. 39 (5), pp. 679-87. Date of Electronic Publication: 2009 Mar 02. - Publication Year :
- 2009
-
Abstract
- Background: Mast cells are important effector cells in innate or acquired immunity that contribute to host defence. Excessive activation of mast cells can result in the development of allergic diseases, including atopic asthma. Mast cell activation by IgE and specific antigen induces the cells to release spasmogenic, vasoactive and pro-inflammatory mediators, which enhance airway smooth muscle contraction, vascular permeability and inflammatory cell recruitment. Recently, we have demonstrated that exposure of mast cells to cigarette smoke medium (CSM) triggered mast cells to produce chemokines. On the other hand, smoking may decrease the risk of allergic sensitization, which could be explained by a reduced IgE production or a diminished response of mast cells to activation of the IgE receptor.<br />Objective: In this study, we investigated the effect of CSM on the allergic activation of mast cells through IgE and antigen.<br />Methods: Primary cultured murine mast cells were exposed to CSM and activated with IgE and antigen or lipopolysaccharide (LPS). The release of granules, production of leukotrienes, chemokines and cytokines was determined in the supernatants by ELISA. The effect of CSM exposure on intracellular signalling, especially the nuclear factor (NF)-kappaB and extracellular signal-regulated kinase (Erk)1/2 pathways, was analysed by Western blotting.<br />Results: CSM suppressed IgE-mediated degranulation and cytokine release, but no effect was observed on leukotriene release. CSM induced phosphorylation of Erk1/2 in mast cells. In CSM-exposed mast cells, activating transcription factor (ATF)-1 was phosphorylated after stimulation with IgE/Ag. LPS-activated mast cells were not influenced by CSM.<br />Conclusion: Our study suggests that exposure to cigarette smoke may lead to a reduced allergic activation of mast cells without affecting their response to activation via e.g. bacterial-derived LPS.
- Subjects :
- Activating Transcription Factor 1 immunology
Activating Transcription Factor 1 metabolism
Animals
Cells, Cultured
Cytokines drug effects
Cytokines immunology
Extracellular Signal-Regulated MAP Kinases immunology
Extracellular Signal-Regulated MAP Kinases metabolism
Leukotrienes biosynthesis
Leukotrienes immunology
Lipopolysaccharides immunology
Male
Mice
Mice, Inbred BALB C
Phosphorylation drug effects
Phosphorylation immunology
Cell Degranulation immunology
Immunoglobulin E blood
Mast Cells immunology
Smoke
Nicotiana immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2222
- Volume :
- 39
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 19260869
- Full Text :
- https://doi.org/10.1111/j.1365-2222.2009.03209.x