Back to Search Start Over

Detection of a cytolytic toxin in the venom of the stonefish (Synanceia trachynis).

Authors :
Kreger AS
Source :
Toxicon : official journal of the International Society on Toxinology [Toxicon] 1991; Vol. 29 (6), pp. 733-43.
Publication Year :
1991

Abstract

The venom of the stonefish, Synanceia trachynis, contains a cytolytic toxin which is antigenic and ammonium sulfate-precipitable, and has a pI of ca 5.7 and an Mr of ca 158,000. The toxin is a potent but narrow-spectrum cytolysin which is lytic in vitro for rabbit, dog, rat, and guinea pig erythrocytes, in that order, but is largely or completely inactive against sheep, cow, human, monkey, mouse, goat, horse, burro and cat erythrocytes. Fractionation of the venom by molecular sieve fast protein liquid chromatography and isoelectric focusing did not separate the haemolytic activity from the venom's lethal and vascular permeability-increasing activities. Also, the three activities were destroyed by heat, proteases, Congo red, potassium permanganate and stonefish antivenoms. The results suggest that the haemolytic, lethal and vascular permeability-increasing activities of stonefish venom are properties of the same molecule, a previously unrecognized, membrane-damaging protein toxin.

Details

Language :
English
ISSN :
0041-0101
Volume :
29
Issue :
6
Database :
MEDLINE
Journal :
Toxicon : official journal of the International Society on Toxinology
Publication Type :
Academic Journal
Accession number :
1926174
Full Text :
https://doi.org/10.1016/0041-0101(91)90065-y