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Effects of PTH treatment on tibial bone of ovariectomized rats assessed by in vivo micro-CT.
- Source :
-
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA [Osteoporos Int] 2009 Nov; Vol. 20 (11), pp. 1823-35. Date of Electronic Publication: 2009 Mar 05. - Publication Year :
- 2009
-
Abstract
- Unlabelled: Using in vivo microcomputed tomography (micro-CT), we found in parathyroid hormone (PTH)-treated osteopenic rats linear increases in cortical and trabecular, due to increased trabecular thickness and number, bone mass. Bone was formed in cavities, leading to restoral of nearly cleaved trabeculae. For the first time, effects in PTH-treated rats were analyzed longitudinally.<br />Introduction: Our aims were to over time (1) determine changes in trabecular thickness and number after PTH, (2) compare responses to PTH between the meta- and epiphysis, (3) determine effects of PTH on mineralization and mechanical properties, (4) determine locations of new bone formation due to PTH on a microlevel, and (5) determine the predictive value of bone structural properties for gain in bone mass after PTH.<br />Methods: Adult rats were divided into ovariectomy (OVX; n = 8), SHAM-OVX (n = 8), and OVX and PTH treatment (n = 9). Between weeks 8 and 14, PTH rats received daily subcutaneous PTH injections (60 microg/kg/day). At weeks 0, 8, 10, 12, and 14, in vivo micro-CT scans were made of the proximal and diaphyseal tibia. After sacrifice, all tibiae were tested in three-point bending.<br />Results: PTH increased bone volume fraction linearly over time in meta- and epiphysis, accompanied by increased trabecular thickness in both and increased trabecular number only in the latter one. CT-estimated mineralization increased in trabecular and remained constant in cortical bone. Ultimate load and energy were increased and ultimate displacement and stiffness unaltered compared to SHAM rats. For those trabeculae analyzed, bone was formed initially on places where it was most beneficial for increasing their strength and later on to all surfaces.
- Subjects :
- Animals
Bone Density drug effects
Bone Diseases, Metabolic diagnostic imaging
Bone Diseases, Metabolic pathology
Bone Diseases, Metabolic physiopathology
Bone Remodeling drug effects
Diaphyses diagnostic imaging
Diaphyses drug effects
Diaphyses pathology
Diaphyses physiopathology
Disease Models, Animal
Drug Evaluation, Preclinical methods
Epiphyses diagnostic imaging
Epiphyses drug effects
Epiphyses pathology
Epiphyses physiopathology
Female
Ovariectomy
Rats
Rats, Wistar
Tibia diagnostic imaging
Tibia pathology
Tibia physiopathology
X-Ray Microtomography
Bone Density Conservation Agents therapeutic use
Bone Diseases, Metabolic drug therapy
Parathyroid Hormone therapeutic use
Tibia drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1433-2965
- Volume :
- 20
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
- Publication Type :
- Academic Journal
- Accession number :
- 19262974
- Full Text :
- https://doi.org/10.1007/s00198-009-0882-5