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Evaluation of a near-senescent human dermal fibroblast cell line and effect of amelogenin.
- Source :
-
The British journal of dermatology [Br J Dermatol] 2009 Jun; Vol. 160 (6), pp. 1163-71. Date of Electronic Publication: 2009 Mar 09. - Publication Year :
- 2009
-
Abstract
- Background: Fibroblast senescence may delay healing of chronic wounds.<br />Objectives: To characterize a chronic human dermal fibroblast cell line (CRL-7815) with near-senescent properties, cell proliferation and production of wound-healing modulating cytokines, and biosynthesis and remodelling of collagen were compared with normal human dermal fibroblasts. Also, the response of CRL-7815 fibroblasts to the extracellular matrix protein amelogenin that is beneficial in the treatment of stalled chronic wounds was studied.<br />Methods: Fibroblast proliferation was monitored by time-resolved growth curves and factors secreted into the culture medium containing 10% fetal bovine serum were measured by enzyme-linked immunosorbent assays. Fibroblast-mediated reorganization was examined in three-dimensional type I collagen matrices.<br />Results: Cell proliferation over 9 days was significantly (P < 0.01) slower for CRL-7815 than for normal fibroblasts. Amelogenin at 1 mg mL(-1) increased (P < 0.01) CRL-7815 proliferation to the level of the normal fibroblasts. The neutrophil chemoattractant interleukin (IL)-8 was low while the constitutive production of monocyte chemoattractant protein (MCP)-1 was highly elevated in medium from cultured CRL-7815 fibroblasts. Amelogenin augmented IL-8 but attenuated MCP-1 secretion in CRL-7815 fibroblasts. The elevated vascular endothelial growth factor production in CRL-7815 fibroblasts was further increased with amelogenin while increased type I collagen synthesis by CRL-7815 was reduced with 0.1 mg mL(-1) amelogenin. The dramatically impaired collagen matrix remodelling with CRL-7815 fibroblasts (P < 0.001) was slightly improved with amelogenin (P = 0.0011).<br />Conclusions: The near-senescent cell line CRL-7815 shares functional anomalies with fibroblasts isolated from nonhealing chronic cutaneous wounds. Amelogenin has the capacity to switch chronic fibroblasts into an acute-like phenotype.
- Subjects :
- Adult
Aged, 80 and over
Area Under Curve
Cells, Cultured
Chemokine CCL2 biosynthesis
Collagen Type I biosynthesis
Female
Fibroblasts drug effects
Humans
Interleukin-8 biosynthesis
Male
Vascular Endothelial Growth Factor A biosynthesis
Amelogenin pharmacology
Cell Line drug effects
Cell Line metabolism
Cell Proliferation drug effects
Cellular Senescence physiology
Fibroblasts physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2133
- Volume :
- 160
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The British journal of dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 19298284
- Full Text :
- https://doi.org/10.1111/j.1365-2133.2009.09071.x