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Low O2 concentrations enhance the positive effect of IL-17 on the maintenance of erythroid progenitors during co-culture of CD34+ and mesenchymal stem cells.
- Source :
-
European cytokine network [Eur Cytokine Netw] 2009 Mar; Vol. 20 (1), pp. 10-6. - Publication Year :
- 2009
-
Abstract
- Co-culture of haematopoietic cells with a stromal cell layer does not mimic the physiological, micro-environmental niche, whose major feature is a low oxygen (O2) concentration. Thus, in order to study the effects of IL-17 in a context which better approximates the physiological state, we investigated its effects on cell expansion, colony-forming ability, and the phenotypical profile of normal, human blood CD34+ cells co-cultured for five days with MSC layers at various O2 concentrations (20%, 12.5% and 3% O2. We demonstrated that IL-17 enhances CD34+ and total CFC production during the five days of MSC/CD34+ co-culture. This effect depends upon the O2 concentration, reaching its maximum at 3% O2, and is more pronounced on erythroid progenitors (BFU-E). In addition, the stimulation of IL-6 production by IL-17 in MSC cultures and co-cultures is enhanced by low O2 concentration. The expression of some differentiation markers (CD34, CD13 and CD41) on haematopoietic cells in co-cultures also depends upon the oxygen concentration. Our results strengthen the concept that physiological levels of O2 (mistakenly called hypoxia), should be considered as an important environmental factor that significantly influences cytokine activity.
- Subjects :
- Antigens, CD34 metabolism
Cell Hypoxia physiology
Coculture Techniques
Colony-Forming Units Assay
Erythroid Precursor Cells cytology
Hematopoiesis drug effects
Hematopoiesis physiology
Humans
Interleukin-6 biosynthesis
Mesenchymal Stem Cells cytology
Erythroid Precursor Cells drug effects
Erythroid Precursor Cells metabolism
Interleukin-17 pharmacology
Mesenchymal Stem Cells drug effects
Mesenchymal Stem Cells metabolism
Oxygen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1952-4005
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European cytokine network
- Publication Type :
- Academic Journal
- Accession number :
- 19318315
- Full Text :
- https://doi.org/10.1684/ecn.2009.0145