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Ectothiorhodospira variabilis sp. nov., an alkaliphilic and halophilic purple sulfur bacterium from soda lakes.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2009 Apr; Vol. 59 (Pt 4), pp. 658-64. - Publication Year :
- 2009
-
Abstract
- During studies of moderately halophilic strains of Ectothiorhodospira from steppe soda lakes, we found a novel group of bacteria related to Ectothiorhodospira haloalkaliphila with salt optima at 50-80 g NaCl l(-1). Phylogenetic analysis using 16S rRNA gene sequences of strains from soda lakes in Mongolia, Egypt and Siberia revealed separation of the group of new isolates from other Ectothiorhodospira species, including the closely related Ect. haloalkaliphila. DNA-DNA hybridization studies demonstrated that the new isolates form a homogeneous group at the species level, but at the same time are distinct from related species such as Ect. haloalkaliphila, Ect. vacuolata, Ect. shaposhnikovii and Ect. marina. The new isolates are considered to be strains of a novel species, for which the name Ectothiorhodospira variabilis sp. nov. is proposed, with the type strain WN22(T) (=VKM B-2479(T) =DSM 21381(T)). Photosynthetic pigments of the novel species are bacteriochlorophyll a and carotenoids of the spirilloxanthin series with spirilloxanthin and derivatives thereof, together with small amounts of lycopene and rhodopin. Gas vesicles are formed by most of the strains, particularly in media containing yeast extract (0.5 g l(-1)) and acetate (0.5-2.0 g l(-1)). Sequence analysis of nifH (nitrogenase) and cbbL (RuBisCO) confirmed the assignment of the strains to the genus Ectothiorhodospira and in particular the close relationship to Ect. haloalkaliphila. The novel species Ect. variabilis is found in soda lakes separated by great geographical distances and is an alkaliphilic and halophilic bacterium that tolerates salt concentrations up to 150-200 g NaCl l(-1).
- Subjects :
- Bacteriochlorophyll A analysis
Carotenoids analysis
Cluster Analysis
Cytoplasmic Vesicles
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Ectothiorhodospira genetics
Ectothiorhodospira physiology
Molecular Sequence Data
Nucleic Acid Hybridization
Oxidoreductases genetics
Phylogeny
RNA, Ribosomal, 16S genetics
Ribulose-Bisphosphate Carboxylase genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Sodium Chloride metabolism
Ectothiorhodospira classification
Ectothiorhodospira isolation & purification
Water Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5026
- Volume :
- 59
- Issue :
- Pt 4
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 19329583
- Full Text :
- https://doi.org/10.1099/ijs.0.004648-0