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Evaluation of aloin and aloe-emodin as anti-inflammatory agents in aloe by using murine macrophages.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2009 Apr 23; Vol. 73 (4), pp. 828-32. Date of Electronic Publication: 2009 Apr 07. - Publication Year :
- 2009
-
Abstract
- The aloe ingredients responsible for physiological effects and the concentrations required to exert their biological activities are not fully understood. This study compares the anti-inflammatory effects of aloin and aloe-emodin with other polyphenols. Our results demonstrated that aloe-emodin dose-dependently inhibited inducible nitric oxide synthase (iNOS) mRNA expression and nitric oxide (NO) production at 5-40 microM. In addition, the levels of cyclooxygenase-2 (COX-2) mRNA and prostaglandin E2 (PGE2) production were suppressed by 40 microM aloe-emodin. Aloin also suppressed the production of NO at 5-40 microM, although it did not suppress PGE2 production. The present results indicate that aloin and aloe-emodin possibly suppress the inflammatory responses by blocking iNOS and COX-2 mRNA expression. The anti-inflammatory effect of aloe-emodin was comparable to that of kaempferol and quercetin, indicating aloe-emodin as a possible key constituent responsible for the anti-inflammatory activity of aloe.
- Subjects :
- Animals
Cell Line
Cell Survival drug effects
Cyclooxygenase 2 genetics
Dinoprostone biosynthesis
Dinoprostone metabolism
Emodin pharmacology
Flavonols pharmacology
Gene Expression Regulation, Enzymologic drug effects
Isoflavones pharmacology
Lipopolysaccharides pharmacology
Macrophages cytology
Macrophages metabolism
Mice
Nitric Oxide biosynthesis
Nitric Oxide metabolism
Nitric Oxide Synthase Type II genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Aloe chemistry
Anthraquinones pharmacology
Anti-Inflammatory Agents pharmacology
Emodin analogs & derivatives
Macrophages drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 73
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 19352036
- Full Text :
- https://doi.org/10.1271/bbb.80714