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Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model.
- Source :
-
Vaccine [Vaccine] 2009 May 11; Vol. 27 (21), pp. 2830-7. Date of Electronic Publication: 2009 Mar 10. - Publication Year :
- 2009
-
Abstract
- Schistosomiasis is an important parasitic disease for which there is no available vaccine. We have focused on a functionally important antigen of Schistosoma mansoni, Sm-p80, as a vaccine candidate because of its consistent immunogenicity, protective potential and antifecundity effect observed in murine models; and for its pivotal role in the immune evasion process. In the present study we report that an Sm-p80-based DNA vaccine formulation confers 38% reduction in worm burden in a nonhuman primate model, the baboon (Papio anubis). Animals immunized with Sm-p80-pcDNA3 exhibited a decrease in egg production by 32%. Sm-p80 DNA elicited specific immune responses that include IgG; its subtypes IgG1 and IgG2; and IgM in vaccinated animals. Peripheral blood mononuclear cells (PBMCs) from immunized animals when stimulated in vitro with Sm-p80 produced appreciably more Th1 response enhancing cytokines (IL-2, IFN-gamma) than Th2 response enhancing cytokines (IL-4, IL-10). PBMCs produced appreciably more spot-forming units for INF-gamma than for IL-4 in enzyme-linked immunosorbent spot (ELISPOT) assays. Overall it appears that even though a mixed (Th1/Th2) type of humoral antibody response was generated following immunization with Sm-p80; the dominant protective immune response is Th1 type. These data reinforce the potential of Sm-p80 as an excellent vaccine candidate for schistosomiasis.
- Subjects :
- Animals
Antibodies, Protozoan immunology
Antigens, Helminth genetics
Cell Line
Chlorocebus aethiops
Cricetinae
Cytokines biosynthesis
Cytokines immunology
Female
Male
Models, Animal
Ovum immunology
Schistosoma mansoni genetics
Schistosoma mansoni metabolism
Schistosomiasis mansoni immunology
T-Lymphocytes immunology
Vaccines, DNA genetics
Antigens, Helminth immunology
Antigens, Helminth metabolism
Fertility
Papio immunology
Schistosoma mansoni immunology
Schistosomiasis mansoni prevention & control
Vaccines, DNA immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 27
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 19366570
- Full Text :
- https://doi.org/10.1016/j.vaccine.2009.02.096