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Effects of transforming growth factor-beta1 and vascular endothelial growth factor 165 gene transfer on Achilles tendon healing.
- Source :
-
Matrix biology : journal of the International Society for Matrix Biology [Matrix Biol] 2009 Jul; Vol. 28 (6), pp. 324-35. Date of Electronic Publication: 2009 Apr 21. - Publication Year :
- 2009
-
Abstract
- Repaired Achilles tendons typically take weeks before they are strong enough to handle physiological loads. Gene therapy is a promising treatment for Achilles tendon defects. The aim of the present study was to evaluate the histological/biomechanical effects of Transforming growth factor-beta1 (TGF-beta1) and vascular endothelial growth factor 165 (VEGF(165)) gene transfer on Achilles tendon healing in rabbits. Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs) were transduced with adenovirus carrying human TGF-beta1 cDNA (Ad-TGF-beta1), human VEGF(165) cDNA (Ad-VEGF(165)), or both (PIRES-TGF-beta1/VEGF(165)) Viruses, no cDNA (Ad-GFP), and the BMSCs without gene transfer and the intact tendon were used as control. BMSCs were surgically implanted into the experimentally injured Achilles tendons. TGF-beta1 distribution, cellularity, nuclear aspect ratio, nuclear orientation angle, vascular number, collagen synthesis, and biomechanical features were measured at 1, 2, 4, and 8 weeks after surgery. The TGF-beta1 and TGF beta 1/VEGF(165) co-expression groups exhibited improved parameters compared with other groups, while the VEGF(165) expression group had a negative impact. In the co-expression group, the angiogenesis effects of VEGF(165) were diminished by TGF-beta1, while the collagen synthesis effects of TGF-beta1 were unaltered by VEGF(165). Thus treatment with TGF-beta1 cDNA-transduced BMSCs grafts is a promising therapy for acceleration and improvement of tendon healing, leading to quicker recovery and improved biomechanical properties of Achilles tendons.
- Subjects :
- Achilles Tendon anatomy & histology
Animals
Biomechanical Phenomena
Bone Marrow Cells cytology
Bone Marrow Cells physiology
Genetic Therapy
Genetic Vectors genetics
Genetic Vectors metabolism
Humans
Male
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells physiology
Rabbits
Transforming Growth Factor beta1 genetics
Vascular Endothelial Growth Factor A genetics
Achilles Tendon physiology
Gene Transfer Techniques
Regeneration physiology
Transforming Growth Factor beta1 metabolism
Vascular Endothelial Growth Factor A metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1569-1802
- Volume :
- 28
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Matrix biology : journal of the International Society for Matrix Biology
- Publication Type :
- Academic Journal
- Accession number :
- 19389474
- Full Text :
- https://doi.org/10.1016/j.matbio.2009.04.007