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GhZFP1, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting with GZIRD21A and GZIPR5.
- Source :
-
The New phytologist [New Phytol] 2009; Vol. 183 (1), pp. 62-75. Date of Electronic Publication: 2009 Apr 15. - Publication Year :
- 2009
-
Abstract
- * Zinc finger proteins are a superfamily involved in many aspects of plant growth and development. However, CCCH-type zinc finger proteins involved in plant stress tolerance are poorly understood. * A cDNA clone designated Gossypium hirsutum zinc finger protein 1 (GhZFP1), which encodes a novel CCCH-type zinc finger protein, was isolated from a salt-induced cotton (G. hirsutum) cDNA library using differential hybridization screening and further studied in transgenic tobacco Nicotiana tabacum cv. NC89. Using yeast two-hybrid screening (Y2H), proteins GZIRD21A (GhZFP1 interacting and responsive to dehydration protein 21A) and GZIPR5 (GhZFP1 interacting and pathogenesis-related protein 5), which interacted with GhZFP1, were isolated. * GhZFP1 contains two typical zinc finger motifs (Cx8Cx5Cx3H and Cx5Cx4Cx3H), a putative nuclear export sequence (NES) and a potential nuclear localization signal (NLS). Transient expression analysis using a GhZFP1::GFP fusion gene in onion epidermal cells indicated a nuclear localization for GhZFP1. RNA blot analysis showed that the GhZFP1 transcript was induced by salt (NaCl), drought and salicylic acid (SA). The regions in GhZFP1 that interact with GZIRD21A and GZIPR5 were identified using truncation mutations. * Overexpression of GhZFP1 in transgenic tobacco enhanced tolerance to salt stress and resistance to Rhizoctonia solani. Therefore, it appears that GhZFP1 might be involved as an important regulator in plant responses to abiotic and biotic stresses.
- Subjects :
- Carrier Proteins genetics
Cell Nucleus
Dehydration
Droughts
Gene Expression Regulation, Plant
Gene Library
Genes, Plant
Plant Diseases genetics
Plants, Genetically Modified
Salicylic Acid
Salt Tolerance genetics
Nicotiana metabolism
Carrier Proteins metabolism
Gene Expression
Gossypium genetics
Kruppel-Like Transcription Factors genetics
Plant Proteins genetics
Stress, Physiological genetics
Nicotiana genetics
Zinc Fingers genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 183
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 19402879
- Full Text :
- https://doi.org/10.1111/j.1469-8137.2009.02838.x