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Cross-sectional characterization of HIV-1 env compartmentalization in cerebrospinal fluid over the full disease course.

Cross-sectional characterization of HIV-1 env compartmentalization in cerebrospinal fluid over the full disease course.

Authors :
Harrington PR
Schnell G
Letendre SL
Ritola K
Robertson K
Hall C
Burch CL
Jabara CB
Moore DT
Ellis RJ
Price RW
Swanstrom R
Source :
AIDS (London, England) [AIDS] 2009 May 15; Vol. 23 (8), pp. 907-15.
Publication Year :
2009

Abstract

Objectives: To characterize HIV-1 env compartmentalization between cerebrospinal fluid (CSF) and peripheral blood plasma over all stages of the HIV-1 disease course, and to determine the relationship between the extent of CSF HIV-1 env compartmentalization and clinical neurologic disease status.<br />Design: Paired blood plasma and CSF specimens were collected from 66 HIV-infected patients cross-sectionally representing all major clinical stages relating to HIV-associated neurologic disease, including primary infection, asymptomatic chronic infection, chronic infection with minor global impairment, and immune deficiency with HIV-associated dementia.<br />Methods: Heteroduplex tracking assays and bulk sequence analysis targeting the V1/V2, C2-V3, and V4/V5 regions of env were performed to characterize the genetic makeup of complex HIV-1 populations in the cross-sectional blood plasma and CSF specimens. The levels of blood plasma/CSF env compartmentalization were quantified and compared across the different clinical stages of HIV-1 neurologic disease.<br />Results: Blood plasma/CSF env compartmentalization levels varied considerably by disease stage and were generally consistent across all three regions of env characterized. Little or no compartmentalization was observed in non-impaired individuals with primary HIV-1 infection. Compartmentalization levels were elevated in chronically infected patients, but were not significantly different between mildly impaired and non-impaired patients. Patients with HIV-associated dementia showed significantly greater blood plasma/CSF env compartmentalization relative to other groups.<br />Conclusion: : Increased CSF compartmentalization of the HIV-1 env gene, which may reflect independent HIV-1 replication and evolution within the central nervous system, is specifically associated with HIV-associated dementia and not the less severe forms of HIV-1 neurologic disease.

Details

Language :
English
ISSN :
1473-5571
Volume :
23
Issue :
8
Database :
MEDLINE
Journal :
AIDS (London, England)
Publication Type :
Academic Journal
Accession number :
19414991
Full Text :
https://doi.org/10.1097/QAD.0b013e3283299129