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Cross-sectional characterization of HIV-1 env compartmentalization in cerebrospinal fluid over the full disease course.
Cross-sectional characterization of HIV-1 env compartmentalization in cerebrospinal fluid over the full disease course.
- Source :
-
AIDS (London, England) [AIDS] 2009 May 15; Vol. 23 (8), pp. 907-15. - Publication Year :
- 2009
-
Abstract
- Objectives: To characterize HIV-1 env compartmentalization between cerebrospinal fluid (CSF) and peripheral blood plasma over all stages of the HIV-1 disease course, and to determine the relationship between the extent of CSF HIV-1 env compartmentalization and clinical neurologic disease status.<br />Design: Paired blood plasma and CSF specimens were collected from 66 HIV-infected patients cross-sectionally representing all major clinical stages relating to HIV-associated neurologic disease, including primary infection, asymptomatic chronic infection, chronic infection with minor global impairment, and immune deficiency with HIV-associated dementia.<br />Methods: Heteroduplex tracking assays and bulk sequence analysis targeting the V1/V2, C2-V3, and V4/V5 regions of env were performed to characterize the genetic makeup of complex HIV-1 populations in the cross-sectional blood plasma and CSF specimens. The levels of blood plasma/CSF env compartmentalization were quantified and compared across the different clinical stages of HIV-1 neurologic disease.<br />Results: Blood plasma/CSF env compartmentalization levels varied considerably by disease stage and were generally consistent across all three regions of env characterized. Little or no compartmentalization was observed in non-impaired individuals with primary HIV-1 infection. Compartmentalization levels were elevated in chronically infected patients, but were not significantly different between mildly impaired and non-impaired patients. Patients with HIV-associated dementia showed significantly greater blood plasma/CSF env compartmentalization relative to other groups.<br />Conclusion: : Increased CSF compartmentalization of the HIV-1 env gene, which may reflect independent HIV-1 replication and evolution within the central nervous system, is specifically associated with HIV-associated dementia and not the less severe forms of HIV-1 neurologic disease.
- Subjects :
- AIDS Dementia Complex complications
AIDS Dementia Complex metabolism
California
Chronic Disease
Cross-Sectional Studies
Disease Progression
HIV Infections blood
HIV Infections complications
Humans
Nervous System Diseases blood
Nervous System Diseases complications
North Carolina
San Francisco
Genes, env
HIV Infections cerebrospinal fluid
HIV-1 genetics
Nervous System Diseases cerebrospinal fluid
Subjects
Details
- Language :
- English
- ISSN :
- 1473-5571
- Volume :
- 23
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- AIDS (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 19414991
- Full Text :
- https://doi.org/10.1097/QAD.0b013e3283299129