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ace, Which encodes an adhesin in Enterococcus faecalis, is regulated by Ers and is involved in virulence.
- Source :
-
Infection and immunity [Infect Immun] 2009 Jul; Vol. 77 (7), pp. 2832-9. Date of Electronic Publication: 2009 May 11. - Publication Year :
- 2009
-
Abstract
- Enterococcus faecalis is an opportunistic pathogen that causes numerous infectious diseases in humans and is a major agent of nosocomial infections. In this work, we showed that the recently identified transcriptional regulator Ers (PrfA like), known to be involved in the cellular metabolism and the virulence of E. faecalis, acts as a repressor of ace, which encodes a collagen-binding protein. We characterized the promoter region of ace, and transcriptional analysis by reverse transcription-quantitative PCR and mobility shift protein-DNA binding assays revealed that Ers directly regulates the expression of ace. Transcription of ace appeared to be induced by the presence of bile salts, probably via the deregulation of ers. Moreover, with an ace deletion mutant and the complemented strain and by using an insect (Galleria mellonella) virulence model, as well as in vivo-in vitro murine macrophage models, we demonstrated for the first time that Ace can be considered a virulence factor for E. faecalis. Furthermore, animal experiments revealed that Ace is also involved in urinary tract infection by E. faecalis.
- Subjects :
- Animals
Bacterial Proteins genetics
Bile Acids and Salts metabolism
Carrier Proteins genetics
Cells, Cultured
Colony Count, Microbial
Enterococcus faecalis physiology
Female
Gene Deletion
Gene Expression Profiling
Genetic Complementation Test
Kidney microbiology
Lepidoptera microbiology
Macrophages microbiology
Mice
Mice, Inbred BALB C microbiology
Virulence
Bacterial Proteins biosynthesis
Carrier Proteins biosynthesis
Enterococcus faecalis pathogenicity
Gene Expression Regulation, Bacterial
Repressor Proteins metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5522
- Volume :
- 77
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 19433548
- Full Text :
- https://doi.org/10.1128/IAI.01218-08