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Interferon-lambda is functionally an interferon but structurally related to the interleukin-10 family.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2009 Jul 31; Vol. 284 (31), pp. 20869-75. Date of Electronic Publication: 2009 May 20. - Publication Year :
- 2009
-
Abstract
- Interferon-lambda (IFN-lambda) is an antiviral cytokine that signals through a distinct receptor complex, composed of the IFN-lambdaR1 and interleukin-10R2 (IL-10R2) receptor chains. We have determined the crystal structure of human IFN-lambda3 and characterized the interaction with its receptor complex through structure-based site-directed mutagenesis. The ability of IFN-lambda3 mutants to signal was determined by measuring the antiviral activity and induced STAT2 phosphorylation. In conclusion, our data show that, although IFN-lambda is functionally an interferon, it is clearly structurally related to members of the IL-10 family. In particular, we found an interesting similarity between IFN-lambda and IL-22, and we suggest that IFN-lambda and IL-22 possess parallel functions, protecting epithelial tissue against viral and bacterial infections, respectively.
- Subjects :
- Antiviral Agents metabolism
Binding Sites
Cell Line, Tumor
Crystallography, X-Ray
Humans
Interferons
Interleukin-10 Receptor beta Subunit metabolism
Models, Molecular
Mutant Proteins chemistry
Mutant Proteins metabolism
Phosphorylation
Protein Binding
Protein Structure, Secondary
STAT2 Transcription Factor metabolism
Interleukin-22
Interleukin-10 chemistry
Interleukins chemistry
Interleukins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 284
- Issue :
- 31
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 19457860
- Full Text :
- https://doi.org/10.1074/jbc.M109.002923