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Elicitation studies in cell suspension cultures of Cannabis sativa L.
- Source :
-
Journal of biotechnology [J Biotechnol] 2009 Aug 20; Vol. 143 (2), pp. 157-68. Date of Electronic Publication: 2009 Jun 11. - Publication Year :
- 2009
-
Abstract
- Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with biotic and abiotic elicitors to evaluate their effect on secondary metabolism. Metabolic profiles analysed by (1)H NMR spectroscopy and principal component analysis (PCA) showed variations in some of the metabolite pools. However, no cannabinoids were found in either control or elicited cannabis cell cultures. Tetrahydrocannabinolic acid (THCA) synthase gene expression was monitored during a time course. Results suggest that other components in the signaling pathway can be controlling the cannabinoid pathway.
- Subjects :
- Analysis of Variance
Cannabis drug effects
Cannabis metabolism
Cell Culture Techniques
Cells, Cultured
Cyclopentanes pharmacology
Dronabinol metabolism
Intramolecular Oxidoreductases genetics
Intramolecular Oxidoreductases metabolism
Metabolome drug effects
Metabolomics methods
Methanol chemistry
Nuclear Magnetic Resonance, Biomolecular methods
Oxylipins pharmacology
Pectins pharmacology
Principal Component Analysis methods
Water chemistry
Cannabis enzymology
Dronabinol analogs & derivatives
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4863
- Volume :
- 143
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 19500620
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2009.05.006