Back to Search Start Over

emm and C-repeat region molecular typing of beta-hemolytic Streptococci in a tropical country: implications for vaccine development.

Authors :
Steer AC
Magor G
Jenney AW
Kado J
Good MF
McMillan D
Batzloff M
Carapetis JR
Source :
Journal of clinical microbiology [J Clin Microbiol] 2009 Aug; Vol. 47 (8), pp. 2502-9. Date of Electronic Publication: 2009 Jun 10.
Publication Year :
2009

Abstract

We designed a study to investigate the molecular epidemiology of group A streptococcal (GAS) and group C and G streptococcal (GCS and GGS) disease in Fiji, a country which is known to have a high burden of streptococcal disease. Molecular typing of the N-terminal portion (emm typing) of the M protein was performed with 817 isolates (535 GAS and 282 GCS/GGS). We also performed genotyping of the C-repeat region in 769 of these isolates to identify J14 sequence types. The profile of emm types for Fiji was very different from that found for the United States and Europe. There were no dominant emm types and a large number of overlapping types among clinical disease states. Commonly found GAS emm types in industrialized countries, including emm1, emm12, and emm28, were not found among GAS isolates from Fiji. Over 93% of GAS isolates and over 99% of GCS/GGS isolates that underwent J14 sequence typing contained either J14.0 or J14.1. Our data have implications for GAS vaccine development in developing countries and suggest that a vaccine based upon the conserved region of the M protein may be a feasible option for Fiji and potentially for other tropical developing countries.

Details

Language :
English
ISSN :
1098-660X
Volume :
47
Issue :
8
Database :
MEDLINE
Journal :
Journal of clinical microbiology
Publication Type :
Academic Journal
Accession number :
19515838
Full Text :
https://doi.org/10.1128/JCM.00312-09