[Construction of standard allelic ladder of miniSTR loci by molecular cloning].

Authors :: Bai X
Cong B
Li SJ
Hou ZP
Gu JL
Liu N
Li X
Guo X
Source :: Fa yi xue za zhi [Fa Yi Xue Za Zhi] 2009 Apr; Vol. 25 (2), pp. 106-8.
Publication Year :: 2009
Abstract: Objective: To investigate the genetic polymorphism of five miniSTR loci (D9S2157, D9S1122, D10S1435, D12ATA63, D2S1776) in Hebei Han population and to construct standard allelic ladders.<br />Methods: Polymorphism of the five miniSTR loci in 120 unrelated individuals was analyzed by fluorescence PCR and ABI 310 Genetic Analyzer. Molecular cloning technique was employed to construct standard allelic ladder of the 5 loci.<br />Results: Of the five miniSTR loci, 8, 8, 7, 5 and 8 alleles were found, respectively. The polymorphism information component were 0.790, 0.720, 0.750, 0.630 and 0.850, respectively.<br />Conclusion: The five loci have relatively abundant polymorphic information and their standard allelic ladders constructed by molecular cloning technique are useful in forensic science.