Back to Search
Start Over
Major role of gamma delta T cells in the generation of IL-17+ uveitogenic T cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2009 Jul 01; Vol. 183 (1), pp. 560-7. - Publication Year :
- 2009
-
Abstract
- We show that in vitro activation of interphotoreceptor retinoid-binding protein (IRBP)-specific T cells from C57BL/6 mice immunized with an uveitogenic IRBP peptide (IRBP(1-20)) under TH17-polarizing conditions is associated with increased expansion of T cells expressing the gammadelta TCR. We also show that highly purified alphabeta or gammadelta T cells from C57BL/6 mice immunized with IRBP(1-20) produced only small amounts of IL-17 after exposure to the immunizing Ag in vitro, whereas a mixture of the same T cells produced greatly increased amounts of IL-17. IRBP-induced T cells from IRBP-immunized TCR-delta(-/-) mice on the C57BL/6 genetic background produced significantly lower amounts of IL-17 than did wild-type C57BL/6 mice and had significantly decreased experimental autoimmune uveitis-inducing ability. However, reconstitution of the TCR-delta(-/-) mice before immunization with a small number of gammadelta T cells from IRBP-immunized C57BL/6 mice restored the disease-inducing capability of their IRBP-specific T cells and greatly enhanced the generation of IL-17(+) T cells in the recipient mice. Our study suggests that gammadelta T cells are important in the generation and activation of IL-17-producing autoreactive T cells and play a major role in the pathogenesis of experimental autoimmune uveitis.
- Subjects :
- Animals
Autoimmune Diseases genetics
Autoimmune Diseases metabolism
Autoimmune Diseases pathology
Cell Communication genetics
Cell Communication immunology
Cell Differentiation genetics
Cell Polarity genetics
Cell Polarity immunology
Cells, Cultured
Coculture Techniques
Disease Models, Animal
Epitopes, T-Lymphocyte administration & dosage
Epitopes, T-Lymphocyte immunology
Eye Proteins administration & dosage
Eye Proteins immunology
Female
Gene Expression Regulation immunology
Interleukin-17 physiology
Lymphocyte Activation genetics
Lymphocyte Activation immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Peptide Fragments administration & dosage
Peptide Fragments immunology
Receptors, Antigen, T-Cell, gamma-delta deficiency
Receptors, Antigen, T-Cell, gamma-delta genetics
Retinol-Binding Proteins administration & dosage
Retinol-Binding Proteins immunology
T-Lymphocytes, Helper-Inducer metabolism
T-Lymphocytes, Helper-Inducer pathology
Uveitis genetics
Uveitis pathology
Cell Differentiation immunology
Interleukin-17 biosynthesis
Receptors, Antigen, T-Cell, gamma-delta biosynthesis
T-Lymphocytes, Helper-Inducer immunology
Uveitis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 183
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 19542467
- Full Text :
- https://doi.org/10.4049/jimmunol.0900241