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Effect of camptothecin on collagen synthesis in fibroblasts from patients with keloid.
- Source :
-
Annals of plastic surgery [Ann Plast Surg] 2009 Jul; Vol. 63 (1), pp. 94-9. - Publication Year :
- 2009
-
Abstract
- Keloids are distinguished by substantial deposition of collagen in the dermis, resulting in an imbalanced production and aggregation of extra cellular matrix. This study was undertaken to evaluate the effects of the topoisomerase I inhibitor camptothecin (CPT) on collagen synthesis in the activated dermal fibroblasts from healthy donors and patients with keloid. The fibroblasts were cultured in the presence or absence of CPT. Cellular toxicity assay was determined by MTT analysis. The expression of type I collagen and type III collagen was studied both at the transcriptional and post-transcriptional levels, using conventional quantitative real-time reverse transcription PCR and Western blotting. Results showed that there was predominantly a clear and dose-dependent decrease in the synthesis of collagen 1, not collagen 3, in keloid fibroblasts without significantly cellular toxicity. The CPT had an activity on the regulation of the ratio of type I/III collagen in the metabolism of keloid fibroblasts by inhibiting the secretion of type I collagen. The data suggest that the inhibitory effect of CPT, a topoisomerase I inhibitor, on collagen synthesis may be an effective treatment for limiting fibrosis in keloid patients.
- Subjects :
- Adolescent
Adult
Blotting, Western
DNA Topoisomerases, Type I genetics
Dose-Response Relationship, Drug
Female
Humans
Keloid genetics
Keloid pathology
Male
Reverse Transcriptase Polymerase Chain Reaction
Skin pathology
Wound Healing drug effects
Young Adult
Camptothecin pharmacology
Camptothecin therapeutic use
Collagen biosynthesis
Collagen drug effects
Enzyme Inhibitors pharmacology
Enzyme Inhibitors therapeutic use
Fibroblasts drug effects
Fibroblasts metabolism
Keloid drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1536-3708
- Volume :
- 63
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Annals of plastic surgery
- Publication Type :
- Academic Journal
- Accession number :
- 19546682
- Full Text :
- https://doi.org/10.1097/SAP.0b013e3181872775