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Merkel cell carcinoma subgroups by Merkel cell polyomavirus DNA relative abundance and oncogene expression.
- Source :
-
International journal of cancer [Int J Cancer] 2010 May 01; Vol. 126 (9), pp. 2240-6. - Publication Year :
- 2010
-
Abstract
- Merkel cell polyomavirus (MCPyV) was recently discovered in Merkel cell carcinoma (MCC), a clinically and pathologically heterogeneous malignancy of dermal neuroendocrine cells. To investigate this heterogeneity, we developed a tissue microarray (TMA) to characterize immunohistochemical staining of candidate tumor cell proteins and a quantitative PCR assay to detect MCPyV and measure viral loads. MCPyV was detected in 19 of 23 (74%) primary MCC tumors, but 8 of these had less than 1 viral copy per 300 cells. Viral abundance of 0.06-1.2 viral copies/cell was directly related to presence of retinoblastoma gene product (pRb) and terminal deoxyribonucleotidyl transferase (TdT) by immunohistochemical staining (p < or = 0.003). Higher viral abundance tumors tended to be associated with less p53 expression, younger age at diagnosis and longer survival (p < or = 0.08). These data suggest that MCC may arise through different oncogenic pathways, including ones independent of pRb and MCPyV.
- Subjects :
- Aged
Aged, 80 and over
Carcinoma, Merkel Cell classification
Carcinoma, Merkel Cell genetics
DNA Nucleotidylexotransferase analysis
Female
Humans
Immunohistochemistry
Male
Middle Aged
Polymerase Chain Reaction
Polyomavirus genetics
Carcinoma, Merkel Cell virology
DNA, Viral analysis
Merkel Cells virology
Polyomavirus isolation & purification
Retinoblastoma Protein analysis
Tumor Suppressor Protein p53 analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0215
- Volume :
- 126
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 19551862
- Full Text :
- https://doi.org/10.1002/ijc.24676